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T-cells & Stem cells

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T-cells & Stem cells

Post  Smurfy on Sun Jun 04, 2017 2:27 am

Hi all,

I came across this and thought it might be worthy of a discussion:

https://www.technologynetworks.com/cell-science/news/regulatory-t-cells-play-essential-role-in-hair-growth-289141
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Re: T-cells & Stem cells

Post  Benjamin Button on Mon Jun 05, 2017 8:25 am

Here's another short and sweet article on this development.

https://www.rdmag.com/article/2017/05/t-cells-could-be-triggers-hair-growth

Immortal, what do you think about this? Have you ever documented "tregs" role in mpb?
Suppressor T cells is what they were called previously. Did you already know about their communication with stem cells?
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Re: T-cells & Stem cells

Post  Smurfy on Sun Jun 11, 2017 1:50 pm

Honestly I'm surprised this isn't getting attention here... it seems like a big development.

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Re: T-cells & Stem cells

Post  Benjamin Button on Sun Jun 11, 2017 8:34 pm

Yes smurfy, I agree, perhaps people are just jaded and can't discern between irrelevant discoveries and very relevant ones like this one..

So I read first article again, and essentially what it boils this immune cell to stem cell communication signal down to is "the jag1 notch signalling pathway"...

So I did a quick Google to see if anybody ever studied jag1 relevance to mpb, and low and behold, this has already been identified in a prior genetic based study as a possible causitive factor in AGA... Wow here's an excerpt

"The expression patterns of Notch signaling pathway genes including Notch 2 and JAG1 were validated by real-time PCR (Figure S1). Jagged1 (JAG1) gene which encodes a ligand for Notch receptor maps to chromosome 20p a susceptibility locus for male-pattern baldness [8]. A reciprocal negative feedback regulation exists between Notch and AR-dependent pathways in the prostate [9]. The activation of AR and the concomitant loss of Notch signaling may be contributing factors to hair follicle miniaturization and may serve as the mechanistic link between prostate cancer and AGA. Thus, modulating the Notch signaling pathway in AGA may lead to future therapies."

That from the journal of dermatological science. "Microarray analysis of androgenetic and senescent alopecia: Comparison of gene expression shows two distinct profiles."

This is pretty god damn interesting... Is this what is really wrong with us? A simple notch signalling error?

CS, surely you have an opinion here? (He's probably researching the shit out of this as we speak! Lol)


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Re: T-cells & Stem cells

Post  shaftless on Mon Jun 12, 2017 2:33 am

Most people can't understand all that research gobblygook terminology. And when it comes down to something going wrong genetically then it's looked upon as nearly impossible to correct. How do you correct genes in a simple manner? Creams? Lotions?

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Re: T-cells & Stem cells

Post  Smurfy on Mon Jun 12, 2017 7:41 am

Benjamin Button wrote:Yes smurfy, I agree, perhaps people are just jaded and can't discern between irrelevant discoveries and very relevant ones like this one..

Very possible... or they browse looking for a quick fix to appear in a thread. Like you said, this is very relevant. Guess we might have to pioneer this one...

Here's something I just came across, speaking of jag1.

http://www.ors.org/Transactions/58/0006.pdf

It seems if we can find a way to locally activate the notch receptor, force signaling, increase the T-cell population locally, activate CD4+ etc, basically immuno-modulate the local environment, we have something here. But what I'm not finding at this point, are natural means in doing so. I haven't come across a study using any extract to mimic what we need. But maybe that link will kick up a few ideas.
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Re: T-cells & Stem cells

Post  Benjamin Button on Mon Jun 12, 2017 8:08 am

Well before going deep, why not just do what they did in the study?

"...replacing Tregs with microscopic beads covered in Jag1 protein restored Notch signaling in the stem cells and successfully activated follicle regeneration."

...all we need to do is get some microscopic beads, cover them with jag1, and then drink them and make a topical. Easy. Lol
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Re: T-cells & Stem cells

Post  Smurfy on Mon Jun 12, 2017 8:16 am

Good idea haha... I knew that little bag of jag covered beads in the fridge would come in handy some day!
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Re: T-cells & Stem cells

Post  Smurfy on Sat Jun 17, 2017 4:07 am

Here's something really good to add to our findings. I found this fascinating...

https://selfhacked.com/2014/11/11/treg/#Increasing_Tregs
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Re: T-cells & Stem cells

Post  Smurfy on Tue Jun 27, 2017 12:41 pm

I would like to call to the witness stand.... IGF-1.

Insulin-like growth factor-1 induces regulatory T cell-mediated suppression of allergic contact dermatitis in mice

Allergic contact dermatitis (ACD) is triggered by an aberrant hyperinflammatory immune response to innocuous chemical compounds and ranks as the world’s most prevalent occupational skin condition. Although a variety of immune effector cells are activated during ACD, regulatory T (Treg) cells are crucial in controlling the resulting inflammation. Insulin-like growth factor-1 (IGF-1) regulates cell proliferation and differentiation and accelerates wound healing and regeneration in several organs including the skin. Recently IGF-1 has also been implicated in protection from autoimmune inflammation by expansion of Treg cells. Here, we demonstrate that ectopic expression of IGF-1 in mouse skin suppresses ACD in a Treg cell-specific manner, increasing the number of Foxp3+ Treg cells in the affected area and stimulating lymphocyte production of the anti-inflammatory cytokine interleukin 10. Similar therapeutic effects can be achieved with systemic or topical delivery of IGF-1, implicating this growth factor as a promising new therapeutic option for the treatment of ACD.

....in the event that Treg activity is a critical factor.
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Re: T-cells & Stem cells

Post  Benjamin Button on Tue Jun 27, 2017 6:28 pm


Interesting
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Re: T-cells & Stem cells

Post  CF Yesterday at 3:43 am

Good stuff in this thread.

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inurl:"thread URL here" site:.immortalhair.forumandco.com/ search term here

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Re: T-cells & Stem cells

Post  Benjamin Button Yesterday at 4:12 am



little bit dead here with little exchange or debate, so I started a thread here...

https://www.hairlosstalk.com/interact/threads/investigating-an-immuno-androgenic-model-of-male-pattern-baldness.106536/
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Re: T-cells & Stem cells

Post  Smurfy Yesterday at 12:51 pm

I read through that whole thread... 75% of it was garbage, ugh. It's baffling how set they are on DHT, and when we get "science-y" with them, they get sarcastic. Like you Ben, I'm here to present what I find one step at a time and generate discussion about the findings. So, I'm going to keep posting more things here, at least it's a cleaner adult conversation.

Here is one very worthy of conversation. This is where things start to get interesting!

Gender And Sex Hormones Influence CD4 Regulatory T Cells And Their Expression Of FoxP3 In Healthy People And In SLE.

Purpose:
The goal of the present study was to determine the role of gender and the effect of sex hormones on the regulation of Foxp3 gene in healthy individuals and in SLE patients.
Methods:
Levels of sex hormones (17 b-estradiol, testosterone), cytokines, and chemokines were measured by ELISA in the serum/plasma of healthy donors and from the culture supernatant of peripheral blood mononuclear cells (PBMC) isolated from SLE patients and healthy controls. PBMC were immunophenotyped by flow cytometry, and mRNA gene expression studies were performed by real time PCR. Protein expression was analyzed by intracellular staining and by Western blot analysis. The in vitro effect of sex hormones 17 b-estradiol (60–100, 500pg/ml), and testosterone (100pg/ml) were studied by culturing sorted CD4+CD25hiCD127lo regulatory T cells for 24–72 hours followed by FACS/Western blot analyses.
Results:
1- The numbers of CD4+ CD25hi Foxp3+ and of CD8+ FoxP3+ regulatory T cells are significantly decreased in healthy females compared to healthy males (p<0.01).
2- Both CD4+CD25+hi and CD8+CD25+ subsets of healthy males had 3–4-fold higher Foxp3 mRNA compared to healthy females.
3- Plasma 17b-estradiol levels are significantly increased in female SLE patients compared to healthy females (p<0.01).
4- The addition of 17 b- estradiol (physiologic cycling luteal phase concentrations of 60 pg/ml) for 24 hours to cultured PBMC from healthy women (but not men) increased the total numbers of cells expressing CD4, CD25 and FoxP3. These effects were not seen in cells in women with SLE.
5- Incubation of CD4+ regulatory T cells with 100 pg/ml of 17b- estradiol maintained FoxP3 expression in SLE women, in contrast to the usual increase in CD4+ Treg that occurs in healthy women at this concentration.
6- Testosterone (100 pg/ml) significantly increased FoxP3 expression in CD4+CD25hi cells from women with SLE. In fact, plasma concentrations of testosterone in those women correlated positively with the expression of FoxP3 in their CD4+CD25+ T cells (p <0.04).
Conclusions:
Women may be more susceptible than men to SLE and other autoimmune diseases in part because many healthy women have fewer regulatory T cells and less FoxP3 expression in those cells. In addition, women with SLE, compared to healthy women, seem to have less ability to generate CD4+ Treg in response to physiologic concentrations of 17 b- estradiol, whereas the testosterone metabolite increases the generation of Tregs. These data suggest that gender and sex hormones may influence susceptibility to SLE via their effects on regulatory T cells.

https://www.blackwellpublishing.com/acrmeeting/abstract.asp?MeetingID=774&id=89904
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Re: T-cells & Stem cells

Post  shaftless Yesterday at 1:19 pm

There was a university in florida or somewhere around there that was experimenting with an igf topical. Very small amounts. They were going to release it after experiments but the results didn't look that dramatic. Not sure if they went ahead and released it anyway or gave up. Igf is nothing new and supposedly you can make it yourself by ingesting soy protein and capsaicin. Sounds too easy. Here's a link.

http://www.hairloss-research.org/UpdateIGF10-11.html

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Re: T-cells & Stem cells

Post  Benjamin Button Yesterday at 11:21 pm

yeah they are mostly assholes

but there are some guys in there who really know their shit, and have backlogues of studies they have researched, you should contribute there too, the initial predictable chaos has died down, the only people posting now seem legit

Working in a team of researchers, mass research, now that was what the internet was made for...

Keep your thread here clean but when you get stuck check over that discussion for avenues you may have missed...
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